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Cellpose+, a morphological analysis tool for feature extraction of stained cell images

24 October 2024
Israel A. Huaman
Fares D. E. Ghorabe
Sofya S. Chumakova
Alexandra A. Pisarenko
Alexey E. Dudaev
Tatiana G. Volova
Galina A. Ryltseva
Sviatlana A. Ulasevich
Ekaterina I. Shishatskaya
Ekaterina V. Skorb
Pavel S. Zun
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Abstract

Advanced image segmentation and processing tools present an opportunity to study cell processes and their dynamics. However, image analysis is often routine and time-consuming. Nowadays, alternative data-driven approaches using deep learning are potentially offering automatized, accurate, and fast image analysis. In this paper, we extend the applications of Cellpose, a state-of-the-art cell segmentation framework, with feature extraction capabilities to assess morphological characteristics. We also introduce a dataset of DAPI and FITC stained cells to which our new method is applied.

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