While pre-training on object detection tasks, such as Common Objects in Contexts (COCO) [1], could significantly boost the performance of cell segmentation, it still consumes on massive fine-annotated cell images [2] with bounding boxes, masks, and cell types for every cell in every image, to fine-tune the pre-trained model. To lower the cost of annotation, this work considers the problem of pre-training DNN models for few-shot cell segmentation, where massive unlabeled cell images are available but only a small proportion is annotated. Hereby, we propose Cross-domain Unsupervised Pre-training, namely CUPre, transferring the capability of object detection and instance segmentation for common visual objects (learned from COCO) to the visual domain of cells using unlabeled images. Given a standard COCO pre-trained network with backbone, neck, and head modules, CUPre adopts an alternate multi-task pre-training (AMT2) procedure with two sub-tasks -- in every iteration of pre-training, AMT2 first trains the backbone with cell images from multiple cell datasets via unsupervised momentum contrastive learning (MoCo) [3], and then trains the whole model with vanilla COCO datasets via instance segmentation. After pre-training, CUPre fine-tunes the whole model on the cell segmentation task using a few annotated images. We carry out extensive experiments to evaluate CUPre using LIVECell [2] and BBBC038 [4] datasets in few-shot instance segmentation settings. The experiment shows that CUPre can outperform existing pre-training methods, achieving the highest average precision (AP) for few-shot cell segmentation and detection.
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